Ploskireva matsakaici (Bacto-agar F): description. Medical ilimin

Ploskireva matsakaici (nan gaba koma zuwa yanzu Bacto agar F) ne a gina jiki matsakaici ga namo wasu kwayoyin, da farko Shigella da Salmonella. Kamar yadda wata majiya ta ga ta yin amfani da kamuwa da kayan: fitsari, bile, tumbi.

labarin

Nikolay Ivanovich Ploskirev - Soviet microbiologist da Karĩmi likita. An haife shi a 1869 ya kuma rasu a shekarar 1948, inda ya yi aiki mafi ransa a garinsu - Tomsk.

A 1888, ya, nan gaba masanin kimiyya, ba tare da kammala shida sa na makaranta, ya shiga soja. ya ya iya kammala horo kawai a 1892, sa'an nan ya wuce da Nazarin ga kudin shiga ga na mallaka Tomsk University, ya yi karatu a Faculty of Medicine. A 1898 ya samu title of likita.

Ploskirev halarci Rasha-Japanese War, da kuma bayan shi aiki a Tomsk asibiti. A 1910 aka nada ga post na shugaban na Tomsk dermatological da venereal asibiti, kuma domin da shekara talatin ya kasance ta dindindin kai. A wannan masanin kimiyya ya kasance daya daga cikin wadanda suka kafa STI asibiti a Tomsk.

Nikolay Ivanovich Ploskirev rubuta jerin ayyukan kishin da yaki da venereal cututtuka.

tsarin

Laraba Ploskireva - abu ga namo na hanji kwayoyin da kuma, sabili da haka, dole ne dauke da nau'i nau'i na gina jiki. An samar a bushe siffan.

Quite babban rabo na yawan nauyi ne pancreatic hydrolyzate sprat (10.4 g / l). Dan kadan kasa lissafta ga hydrocitrate dibasic sodium (8.5 g / l). Har ila yau, akwai wata bushe gina jiki broth da kuma madara da sukari (8.62 da kuma 7.3 g / l).

The title na biyu Ploskireva matsakaici - J. Bacto-agar a cikin abun da ke ciki shi ne ba a wani adadin agar, 6,94 g / l. Seriovatistokislogo anhydrous sodium ciki daidai 5.1 g / l. Akwai gaban anhydrous disodium phosphate - 2.1 g / l, sodium salts na cholic acid game da 3,46 g / l kuma soda ash - 2.4 g

Kasa da gram žunsa Manuniya - tsaka tsaki ja (0.05), kuma m kore (0.0002). A aidin abun ciki - 0,13.

aikace-aikace

A binciken da na biochemical matakai na kwayoyin taka sosai muhimmiyar rawa a cikin ganewar asali da cututtuka da suka haddasa anaerobic kwayoyin. Species a cikin wannan iyali - daruruwan. Su ne kusan m, a ilimin halittar jiki da kuma al'adu Properties, kuma mafi m hanyar bambanta su daga juna - shi ne nazarin Biochemistry.

A binciken da farawa da inoculation na pathological abu a cikin al'adun matsakaici. Ta pathological bambanci ga coliform kwayoyin da kuma wani ɓangare na shi, ban da bayyana agar, ya hada da haske da kuma lactose. Processing ikon da lactose - wani muhimmin alama na bambantawa na enterobacteria. Idan wannan aka gano, da PH canjawa zuwa acidity da nuna alama ke kunne, wanda ya jũya mallaka.

Wasu ƙasashe na iya amfani da sauran gina jiki kafofin watsa labarai, amma da tsarin ba da aikin yayi dace da uku ya fi na kowa a Rasha - wannan yanayi Endo da Levine Ploskireva Laraba.

Description na hanyar

Kasashen waje Ploskireva analog matsakaici ne ake kira Macconkey Agar. A ƙãre form m bayani yana mai haske ruwan hoda-yellow launi. Mazauna iya aiwatar lactose, tsakanin Ploskireva ba ja (Cranberry) launi. Idan kwayoyin ba zai iya aiwatar da shi, ko mallaka ne colorless ko yana da wani rauni launi.

Ganin cewa abun da ke ciki na matsakaici ya hada abubuwa Ploskireva hanawa (m kore, bile salts, aidin), shi ne kusan gaba daya damuarn ci gaban da gram-tabbatacce Flora, kamar yadda a farkon yini eshehiry muhimmanci retards da girma, kazalika da sauran saba concomitant microflora.

biyu kafa

Bugu da ari ban sha'awa shi ne zabin na mallaka, kuma shi ne seeded a kan wani primary ajiya matsakaici da kuma bambantawa. Yanayi a cikinsa akwai wata seeding kamata dauke da mahara substrates. Kwayan al'ada kamata gane enzymatic aiki tare game da su, to wannan matsakaici sanya shi a cikin shambura haka cewa akwai biyu shafukan:

• daya a cikin abin da slanting agar.
• tare da wani shafi.

The ban sha'awa da masu bincike a cikin mallaka lugar bevelled rabo mai yawa bugun jini da kuma a cikin wani shafi - a prick. A wannan mataki, da matsakaici amfani Russell, Kligler ko Olkenitskogo. Kamar yadda bambanci ganewar asali amfani da Ploskireva Laraba.

Ilimin da pathogenic kwayoyin

Agar Ploskireva ne wani muhimmin factor a ganowa cututtuka sa ta Enterobacteriaceae. Saboda haka, misali, shi ne girma da kuma bambanta mazauna na kwayoyin wanda sa bacillary dysentery. Wannan anaerobic kwayoyin na ga HALITTAR Schigella.

Kamar duk wanda shi ne wani ɓangare na wani iyali na hanji kwayoyin cuta, shigella da nau'i na sandunansu, girman biyu micrometers. Ba su dokoki ne capsules da spores da flagella - shi damar da su zuwa a iya bambanta daga Salmonella, wadda, bi da bi, su ne mobile. Suna girma sosai a kan mafi sauki kafofin watsa labarai, a zazzabi dan kadan sama na yanayi (35-37 ° C) da kuma 7,4 PH. By da yanayin girma, da mazauna ba su bambanta ba daga Salmonella.

Kamar yadda aka tattauna a sama, kwayoyin iya zama ma morphological bambance-bambance, duk da haka, ya bambanta muhimmanci a biochemical tafiyar matakai na rayuwa. Saboda haka, sai dai Shigella Nyukestl, ba tare da samuwar acid gas na faruwa a lokacin fermentation na carbohydrates. Da ban da Shigella sonnei, ba za su iya ferment lactose, amma an narkewar glucose. Just to su key fasali, za ka iya kawo musu abin da za su iya wajen rage niraty zuwa nitrite. Su mazauna aka narkewar da urea, kazalika a cikin al'adun matsakaici ne babu liquefaction na gelatin.

Tarin kuma shirye-shiryen da iri

Don gane da jamiái na dysentery, ana buƙatar microbiological gwaje-gwaje infested muhallin, watau faeces haƙuri. A kan samu daga cikin abu, kana bukatar ka da sauri a yi da shuka. Idan wannan shi ne ba zai yiwu, Madogararsa dole ne a sanya a cikin wani hana aifuwa na maza - phosphate buffer cakuda ko glycerol. A 4 ° C, su za a iya adana fiye da wata rana. Tattara da kayan dole ne a za'ayi ta wajen wani rectal gilashi tube wanda aka saka a cikin dubura.

Domin nazarin da wani abu zaba surkin jini gamsai guda da za a wanke a biyu zuwa uku shambura isotonic sodium chloride bayani.

Aikace-aikace na pathogenic kwayoyin cuta a cikin yanayi Ploskireva sanya gilashi spatula. Kana bukatar ka Rub a kan wani karamin sashi na ta agar, sa'an nan yaga kashe spatula a kan muhalli, da kuma saura abu ne shafa a cikin sown surface. Idan seeding aka yi a 'yan kofuna, sa'an nan zuwa ga kowane daga cikinsu bukatar dasa wani sabon tsari.

Idan muco-surkin jini guda akwai a shan a kan wani kwazo analysis, shi ba ya nufin cewa babu gaban pathogens. A irin wannan yanayi wajibi ne a emulsify tumbi a 10 ml na sodium chloride (maida hankali 0,85%) sa'an nan shuka daya ko biyu saukad a kan wani matsakaici Ploskireva. Non-emulsified kujera za a iya sown a selenitovy broth. Ya kuma yi amfani da, idan, maimakon tumbi ne aman ko wanke-wanke.

microbiological ganewar asali

A mataki na farko na microbiological nazarin za'ayi shuka na pathogenic kwayoyin cuta a cikin biyu kofuna, sa'an nan zuwa watch yadda za girma Shigella. A ranar Laraba Ploskireva samar da amfanin gona, da kuma na biyu - a kan yanayi ko Endo Levin.

Saboda gaskiya cewa akwai damuwa shigella suna resistant maganin rigakafi, da matsakaici da aka supplemented da chloramphenicol. Sa'an nan da shi ya auku a cikin kwanaki shiryawa a wani incubator a 37 ° C.

A rana ta biyu don gano da girma mazauna. Wadanda cewa ba a kan bambanci bincike matsakaici girma colorless, shi wajibi ne don sako daga Russell ta matsakaici ko short "motley jere." Bugu da ari bincike da ake gudanar da algorithm na farko da amfanin gona a kan su. Mazauna na Shigella a ranar Laraba Ploskireva girma a cikin nau'i na m, colorless da kananan ginshikan. Su na iri biyu:

• mĩƙe tare da jagged gefuna.
• taso, convex, tare da hankula rigar haske.

Uku ko hudu mazauna dole ne a mikroskopirovat kuma yayi nazari domin motsi. Idan karshen ba ya nuna sama, suka koma a kan Laraba Olkenitskogo don ware wani m al'adu. A cikin rashi na halayyar mazauna Shigella ko idan babu ci gaban da aka lura a duk, shi wajibi ne don Endo agar ko Ploskireva kayan seeding na selenitovogo broth. Idan da hankula mazauna ne ba a isa lambobi, sa kiyasta agglutination. A cakuda Sera sonnei da Flexner, dauki auku a kan gilashin.

arin bincike

A rana ta uku akwai canje-canje da suka faru a da mazauna a kan matsakaici Russell ta. Idan akwai wani al'ada cewa ba kaskantar da lactose kuma glucose ferments, don samar da wani acid, shi ne rabu da bincikar, sai ku ciyar bincike da madubin likita, kazalika da gudanar da shuka a cikin "motley jere," amma wannan lokacin kumbura. Just sa da agglutination dauki, domin serological ganewa.

A rana ta ƙarshe, shi za a iya kammala a kan tushen da canje-canje a cikin "motley yawan" (idan akwai fermentation na carbohydrates), kazalika da takaita agglutination dauki.

Storage da kuma shirye-shiryen

Shiri na agar Ploskireva auku kamar haka:

1. 55 g bushe abu da aka zuga a wani lita na distilled ruwa.
2. Boiled biyu zuwa uku minti har da agar ba tukuna gaba daya narke.
3. Zuba a cikin Petri yi jita-jita (sterility dama) Layer na 5-6 mm.
4. A faranti an bar bude ga wani rabin awa a dakin da zazzabi (18-25 digiri). Bayan wannan lokaci da matsakaici ne isasshe taurara da kuma bushe.

Laraba Ploskireva photosensitive da hygroscopic. A foda kamata a adana a cikin shãfe haske kunshin, da dangi zafi a cikin dakin dole ba wuce 60%. A ganiya zazzabi ga ajiya na 2 ° C zuwa 25 ° C. Storage dole ne bi da wadannan dokoki, in ba haka ba zai yiwu m binciken.