Vitrification na tãyuna a cikin mutum dako

A wannan labarin, za mu yi magana game da irin wannan ra'ayi kamar yadda vitrification na tãyuna. Dr Kuwayama Masashige da aka kirkiro wannan hanyar don kriotopah ko a cikin shekara shekara. A farko yaro haife ta hanyar vitrification tãyuna a cikin 2003. oocyte rayuwa kudi da aka karu da 98 bisa dari.

Rabin da mata da suka sami IVF, da tãyuna kasance. Domin su gudanar cryopreservation da damar marasa lafiya domin ya ceci kudi. Bayan duk unfreeze da kuma canja wurin da tãyuna ne sauƙin fiye da a gudanar da wani a cikin vitro hadi hanya sake. Shi ne kuma wani irin inshora a harka idan wata mace ba ciki. Cryopreservation yana da wani jinsin amfani - hana mutuwar mai yiwuwa embryos wanda ya kasance bayan da yarjejeniya.

yanayin halitta na juya

Domin da kayadadden ci gaban da kwayoyin, ko yanayin halitta na juya, shi Dates baya daga lokacin hadi da kuma ƙare da mutuwarsa. Wannan motsi ne m, a lokacin da yana da wani m hali. Kuma ba za mu iya ya dakatar ko rage gudu ta ci gaba. Amma halitta akwai ban. Waɗannan su ne tsire-tsire, kwaro da kuma ma wasu na farko vertabrate cewa a low zazzabi, ba nuna kaddarorin halayyar rayayyun kwayoyin halitta.

Mene ne rashin himma?

Vitrification na tãyuna tattauna a kasa. Mutane daya-daya lokaci na kwantar da hankula da aka kira rashin himma. Alal misali, mutane da yawa dabbobi tsira da Siberian yanayin zafi kai -90 digiri, da kusan cikakkiyar dehydration. A cikin nazarin wannan lokaci na ontogenesis a cikin yanayi akwai wata tambaya game da yiwu amfani da low yanayin zafi ga m da kuma reversible katsewa na aiki na sama vertebrate halittu, ciki har da mutane.

cryopreservation

Cryopreservation - shi ne wani tasiri Hanyar domin dakatar da nazarin halittu tafiyar matakai a Kwayoyin da daukan hotuna zuwa low zazzabi. Wannan ya tserar da ran aiki na sel a lokacin dumama. By shahararsa, wannan hanya ne na baya tayi vitrification. 1 kriotop (labeled krionositel) ya ƙunshi daga 1 zuwa 3 gudan jini.

Alal misali, lokacin da yin wani hanya kamar IVF, mafi kyau sakamako ne dauke da za a motsi a cikin igiyar ciki rami ba fiye da biyu gudan jini. Sauran ingancin tãyuna za a iya cryopreserved domin nan gaba amfani. Su kuma iya tambaya domin sake IVF bayan wani lokaci, idan hanya za ta nuna wani mummunan sakamako. Tare da irin wannan a raga kwashe vitrification tãyuna a kan mutum dako.

A wasu lokuta, daskare duk gudan jini. Domin matan da suka yi ovarian hyperstimulation ciwo a lokacin superovulation shigar da aka yi mafi sau da yawa. Wanda ya bada shawarar daskarewa? Marasa lafiya fama da ciwon daji, musamman kafin hanya jiyyar cutar sankara ko radiotherapy. A data aka sa'an nan kuma canja tãyuna a cikin igiyar ciki rami. Daskare show duk wanda dama a ciki bayan IVF saboda wasu dalilai saukar da. Wannan na iya zama endometrial polyps, kasa endometrial kauri zuwa lokaci shirya canja wuri, dysfunctional zub da jini.

daskarewa, saukarwa

A tãyuna aka daskarewa a daban-daban, saukarwa:

• hadu kwai (zygote).
• crushing mataki tãyuna.
• blastocysts.

A lokacin, akwai hanyoyi guda biyu na misãlin gudan jini.

m daskarewa

Vitrification na tãyuna ne da za'ayi a wata m daskarewa. Irin wannan hanyar da aka samarwa a cikin 70s da kuma daya daga cikin na farko na gargajiya da hanyoyin da misãlin gudan jini. Yana dogara ne a kan jinkirin sanyaya a akai kudi. Da zarar jinjirin da aka adana a cikin ruwa nitrogen.

Amma ya kamata a lura da cewa a jinkirin daskarewa a cryoprotective bayani microscopic kankara lu'ulu'u suna kafa wanda adversely shafi sel amfrayo. Yana iya sa m ko duka lalata nazarin halittu abu a kan dumama. Yawan tãyuna nasarar canjawa wuri a lokacin jinkirin daskarewa da kuma thawing, kamar kashi 70 cikin dari.

vitrification

Bayan shekara ta 2010, ya fara amfani da wani sabon da kuma mafi inganci Hanyar cryopreservation - vitrification. Idan aka kwatanta da baya hanya, wannan shi ne wani nafani hanya zuwa daskare nazarin halittu abu. Mafi sau da yawa dauki tãyuna bayan vitrification PGD (kayyade ganewar asali).

Lokacin amfani da wannan hanya cryoprotective bayani, inda tãyuna an sanya ba ya samar da kankara lu'ulu'u a lokacin misãlin. Saboda haka, rage outage yiwuwa tayi. A fifiko na wannan hanya shi ne ba wai kawai wata hanya daskarewa, amma kuma da rayuwa kudi na tãyuna bayan thawing. Bisa kididdigar da, yawan tsira tãyuna bayan vitrification tsari ba kasa da kashi 95%.

Abin da ya faru bayan thawing?

Bayan thawing da tãyuna ba su bambanta ba daga talakawa gudan jini. Su da kai tushen da kuma girma. By dumama duk tãyuna hõre da taimaka Hatching. A gudanar da wannan aiki cikin surface Layer amfrayo da aka raba Laser katako a da ake so kwana da lafiya. Wannan facilitates tayi daga cikin harsashi, kuma qara da yiwuwar nasara dasawa a cikin mahaifa.

Misãlin tãyuna damar ajiya na dogon lokaci. Wannan tsari ne kudin-tasiri, saboda farashin adana, thawing da implanting da tayi a cikin mahaifa ne kasa da wata biyu aiwatar da a vitro hadi.

Vitrification aka dauke a matsayin phased mika mulki inda sanyi bayani a karkashin sanyaya kasa da gilashin mika mulki zazzabi. A lokaci guda shi ya zauna amorphous tsarin da gilashi da kuma samun wani ingancin kama crystalline daskararru. Saboda haka, duka biyu da rai Kwayoyin, har ma da dukan tayi an canza kama zuwa "gilashin". Idon yayi kamar gilashi tsarin da ruwa da aka samu a karkashin vitrification saboda ta m sanyaya, Ina nufin entropy daga cikin ruwa an rage a cikin wani guntu lokaci fiye da zama dole entropy crystal tsarin.

A sauki kalmomi, da ruwa ba ya daskare idan ta entropy tsarin kula da tabarbarewa crystal. Amma domin yadda ya kamata vitrified rai gabar, shi wajibi ne don cimma wani kudi na zazzabi karkatar ≈ 108 ° C / min., Kuma ba shi yiwuwa a yi, saboda amfani cryogenic ruwa ne bai isa ba, domin wannan zafin jiki, da kuma ba za a iya amfani da vitrifitsiruemy bayani a wani karami girma fiye da ƙarar oocyte. Wannan duk da shawara da kuma vitrification na tãyuna. Mene ne shi, shi ne a yanzu fiye ko žasa bayyananne.

Masana kimiyya sun iya tabbatar da cewa da karuwa a ranar Laraba zuwa daskare cryoprotectants ba ka damar da sauri runtse daskarewa gudun. Saboda haka, a wata yawa na 10% propylene glycol da etilengligolya gudun aka ƙwarai rage, a 40 bisa dari na da yawa daga vitrification ne zai yiwu a sanyaya kudi na 10 ° C / min, kuma a 60% kudi saukad da zuwa 50 ° C / min. Amma tare da karuwa a yawa daga cryoprotectants, fadowa a ranar Laraba, kara da mummunan tasiri a kan daskarewa biomaterials. Slow daskarewa tsokani jari na chilled ruwa a nazarin halittu kwayoyin da kwayuka kashi. Irin wannan yanayin ya auku saboda tsananin dehydration Kwayoyin tare da bayyanar da extracellular kankara.

Haka kuma, a kan samu vitreous Tsarin karya sinadaran da jiki tafiyar matakai na dehydration. Duk da cewa da vitrification na tãyuna (cewa shi ne, an bayyana su daki-daki a bisa) shi ne m wuya jiki tsarin, kayan da wannan tsarin za a iya samu a rayuwar yau da kullum (gilashi, silicon, kuma haka a).

Vitrification na tãyuna: reviews

Wannan hanya tattara kawai tabbatacce feedback. vitrification hanya ne m. Amma yana da wani yawa fasali a daban-daban matakai na ci gaba a IVF dakunan gwaje-gwaje. Vitrification - ba wani sabon Hanyar cryopreservation rayuwa Kwayoyin. Shi ne na karshe mataki na jinkirin daskarewa. Yau, da yawa mata suna iya samun wani jariri saboda kimiyya aukuwa.

binciken

Saboda aikin masana kimiyya da dama vitrification za a iya yi ba tare da yin amfani da tsada kaddara injin daskarewa da kuma yin amfani da sauki kayan aiki da kula da tafiyar da aiki. Saboda haka, Sauki Hanyar da wani ingantaccen sakamako. Duk da babban ci gaba a cryopreservation, da aiwatar da ya dace kiyayewa rayayyun kwayoyin halitta a low zafin jiki a yau shi ne ba zai yiwu ba.