TLC sauki hanyoyin hadaddun analysis

TLC ne shugaba a al'amurran da suka shafi na gwada yawa da kuma Semi-gwada yawa bincike na hadaddun Pharmaceutical, na halitta, ilimin halittu da aikin likita, fasaha, da kuma sinadaran da yawa wasu abubuwa. Thin Layer chromatography ne ma mafi m Hanyar taro bincike na kusan duk azuzuwan abubuwa.

Wannan dabara da rabuwa da kuma bincike na Multi-bangaren tsarin da ake amfani da ko'ina a dakunan gwaje-gwaje na sosai daban-daban bayani dalla-dalla da kuma shugabanci: tsafta-ANNOBA, dabbobi, agrochemical, bincike, na bincike, kuma domin shuka kariya tashoshin, Center for Standardization da ofishin na bincike Medicine.

Babban abũbuwan amfãni daga bakin ciki-Layer chromatography, dangane da tashin eluent (analyte sauran ƙarfi) a cikin sorbent Layer ta hanyar da haka ya kira capillary sojojin ne da sauki da kuma easiness na chromatographic tsari, da babban mataki na daidaici na bincike da kuma gwada low cost da zama dole kayan aiki.

TLC, da ciwon babban mataki na ji na ƙwarai (ƙananan ganewa dokin) da kuma selectivity, damar domin sanin 10-20 ug abubuwa har zuwa 7%, wanda shi ne sosai high. Thin Layer chromatography iya zama adsorption da kuma rarraba iri. A mafi yadu amfani ne da farko wani zaɓi.

Jigon da hanyar da yake a cikin TLC sauran ƙarfi abu motsa a cikin sorbent gado da kuma sarari rarraba da daban-daban aka gyara bisa ga mutum halaye da dukiyoyinsu. Gaskiyar cewa kwayoyin na daban-daban abun da ke ciki da kuma tsarin da adsorbed (ajiye) a kan surface na m chromatographic farantin daban. Saboda haka, hadaddun Multi-bangaren tsarin da ake hankali rabu cikin mutum aka gyara.

Motsi a daban-daban gudu tare da sorbent gado, da mutum an riga an rabu, da aka gyara ta samar da wata chromatogram. Kowane launi tabo yayi dace da takamaiman sinadaran. Colorless abu da ultraviolet da haske a ƙarƙashin rinjayar da wani jam'i na mahadi fara fluoresce, ko hõre musamman magani canza launi reagent.

Daga cikin zamani hanyoyin bincikar GLC tsaye a waje. Wannan hanya ne halin da rabuwa da kuma bincike ta amfani da, a matsayin sorbing wakili na musamman nonvolatile ruwa, cikinsa da powdered yumbu abu ne wetted. Kuma kamar yadda na tuki matsakaici amfani a nan daban-daban inert gas. Wannan hanya ita ce mafi tasiri a nazarin hadaddun gaurayawan dake dauke da daruruwan daban-daban aka gyara.

Wani zamani hanya domin gudanar da bincike da abun da ke ciki na hadaddun abubuwa ne ruwa chromatography. A babba alama ce ta wannan Hanyar kunshi a cikin rabuwa da mutum aka gyara ta hanyar yin amfani da bambance-bambance cikin rarraba tsakanin m (eluent) da kuma gyarawa (adsorbent) bulan. Wannan hanya da aka fi sau da yawa amfani a cikin bincike na daban-daban Organic mahadi (abinci Additives, da magungunan kashe qwari, daban-daban da gubobi, da dai sauransu). Har ila yau, irin wannan chromatographic Hanyar da aka yi amfani da su yi nazarin da ingancin iko da Pharmaceutical kayayyakin, tabbatar da dalilin da steroids a nazarin halittu ruwaye, da kuma sauran amino acid analysis.