Mene ne kyamara Goryaeva? Dokokin na jini Kwayoyin kirgawa

Yau Goryaeva kyamara shi ne daya daga cikin mafi used na'urorin a dakin gwaje-gwaje bincikowa. Ana iya amfani da wajen ƙidaya yawan daban-daban da maikacin jini. Kamar yadda aka sani, a jini gwajin ne da muhimmanci sosai ga aiwatar da bincike da kuma lura da kusan a kowace cuta.

Mene ne kyamara Goryaeva?

Irin wannan sanannun da kuma amfani da na'urar da aka ɓullo da kuma samarwa Rasha likita N. K. Goryaevym. Goryaeva kamara, a gaskiya, shi ne wani takamaiman gilashin nunin mai rufi da microscopic raga.

Me amfani da wannan kyamara? Da farko shi iya taimaka ƙayyade yawan jini Kwayoyin, musamman leukocytes da erythrocytes. Ba zato ba tsammani, tare da taimakon shi ne mai yiwuwa a ƙayyade da karuwa daga cikin microscope amfani. A dabara domin wannan shi ne kamar haka:

• Kg = (m2 - M1) / An

A wannan yanayin:

• Kg - karuwa daga cikin microscope.
• m2 - da matsayi na dama iyaka Goryaeva kyamara cell.
• M1 - da matsayi na bar iyakarsu.
• da kuma - da size na sel guda, wanda shi ne wani misali da shi ne 0.05 mm;
• N - shi ne yawan Kwayoyin tsakanin auna jam'iyya iyakoki.

A tsarin da kyamara

Goryaev kyamara shi ne kome innoe a matsayin gilashi slide (amma da yawa thicker fiye da saba) raba da su kẽta GROOVES cikin sassa uku. A tsakiyar ɓangare na gilashi ƙunshi Grid domin kirgawa. A karshe na wannan jam'iyya amfani ga man shafawa coverglass - haka, mai tsaron da aka halitta rufaffiyar jam'iyya da capillary sarari a tarnaƙi, kuma ta hanyar da aka cika da ruwa.

Amma ga Grid, da Goryaeva jam'iyya ne zuwa kashi 225 manyan murabba'ai daidai size - suke located in goma sha biyar layuka. 25 manyan murabba'ai an kara zuwa kashi karami, 16 a cikin kowane. A tsawon kowane gefe na kananan square ne 0.05 mm.

Shirye-shirye lissafi na kafa abubuwa

Hakika, a cikin wannan yanayin, shi ne muhimmanci dabara na dakin gwaje-gwaje jarrabawa. Hakika, duk Goryaeva jam'iyya saman ya zama mai tsabta da kuma bushe. Bayan haka, murfin gilashin niƙa sabõda haka, kana iya ganin hali mai haske zobe. Yana da muhimmanci cewa jam'iyya da aka ba iska kumfa, kamar yadda wannan na iya karkatar da sakamakon bincike.

Babu shakka, domin ƙidaya yawan corpuscles, daban-daban reagents aka yi amfani ga kowane iri-iri na jini Kwayoyin. Alal misali, amfani da kirgawa erythrocytes 0.9% sodium chloride bayani. A vitro Mix 8 ml gishiri bayani da kuma 0.02 ml na jini. Saboda haka, dakin gwaje-gwaje dilutes da jini 400 sau. Wani lokaci waddan iya zama mai girma.

Domin ƙidaya yawan leukocytes, shi wajibi ne don Mix 0.4 ml na acetic acid (shan 3% ko 5% bayani) da kuma 0.02 ml na jini.

Bayan da sinadaran gauraye a wani gwajin tube, ta amfani da musamman pipetting samun wani karamin adadin daga cikin cakuda da hankali cika masallaci ga wani asusu (yawanci, daya ko biyu saukad da zai zama isa).

Yadda za a lissafta yawan jini Kwayoyin?

Kirgawa na ja da maikacin jini a Goryaeva jam'iyya ne da za'ayi a cikin biyar manyan murabba'ai, wanda shi ne daidai da tamanin kananan. Domin kauce wa kurakurai saboda da m rarraba jini samfurori, zabi cikin murabba'ai shirya diagonally. Musamman hankali ne ya biya zuwa Kwayoyin located a kusa da gefuna - suna dauke ja jini Kwayoyin a kan hagu da kuma saman ganuwar, amma ba ya yi la'akari da waɗanda suke a cikin kasa da kuma dama Lines.

A don ƙayyade yawan ja jini Kwayoyin da milliliter na jini, da yawan Kwayoyin a biyar manyan murabba'ai ta tara da 20 000 (idan diluted 400 sau).

Leukocytes a cikin masallãci Goryaeva zaton daban. Ga shi wajibi ne yin lissafi da yawan abubuwa na akalla wani mutum ɗari manyan murabba'ai. A sakamakon yawan da aka raba ta 1600, sa'an nan ta tara da 4000, sa'an nan ta 20 (dilution).