Recombinant erythropoietin. Recombinant mutum erythropoietin

Erythropoietin (EPO) - glycoprotein hormone cewa iko da kudi na erythropoiesis a cikin mutane. A halin yanzu fili an hada da farko a cikin kodan, karamin adadin (game da 10 bisa dari) da aka samar a cikin hanta. A hormone erythropoietin activates da division da bambantawa na erythroid magabatansu. Matsayin na ciki hormone a cikin jini jini na lafiya mutane dabam a cikin wani m kewayon kuma shi ne inversely na gwargwado ga haemoglobin taro da kuma mataki na oxygenation na kyallen takarda. Aiki a kan warewa da kuma samar da wannan hormone domin magani ne da amfani sosai.

Tsari da kuma darajar hormone

Jigidar halittar kunshi amino acid hormone. A rashi na na ciki erythropoietin ya kasance mai kaifi karu a matakin na haemoglobin da kuma ja da maikacin jini, shi yana bunkasa da ake kira eritropoetindefitsitnaya anemia. zauna ba zai yiwu ba saboda rashin dace pharmaceutical Har kwanan nan, irin wannan magani gyara na anemia. Yau, tare da kasawa sama da hormone a cikin jikin mutum, likitoci rubũta recombinant erythropoietin. A shirye-shiryen samu daga dabbobi Kwayoyin a cikin abin da ya gabatar da gene code for mutum EPO. Recombinant mutum erythropoietin a amino acid da kuma carbohydrate abun da ke ciki shi ne m ga halitta hormone, qara yawan ja da maikacin jini, reticulocytes, activates da biosynthesis na haemoglobin a cikin sel. A nazarin halittu aiki na da sakamakon abu ne ba daban-daban daga na ciki hormone. Recombinant erythropoietin ba nuna cytotoxic effects kuma ba zai tasiri a leucopoiesis. Masana kimiyya bayar da shawarar cewa EPO interacts tare da takamaiman eritropoetinchuvstvitelnymi rabe, wanda aka located a kan salula surface.

A tsari ga tsarkakewa recombinant mutum erythropoietin

Recombinant mutum EPO - shi ne daya daga cikin mafi yawan sunadaran da suka samu ta hanyar yawa nazarin halittu da kuma pharmaceutical kamfanonin duniya domin miyagun ƙwayoyi far. A halin yanzu fili an hada kwai Kwayoyin Sin hamster (Cho) da recombinant DNA dabaru. Daya polypeptide sarkar recombinant EPO ƙunshi 165 amino acid, da lasafta kwayoyin nauyi na 24,000 Yeah, da kuma lura da kwayoyin nauyi na glycosylated gina jiki - 30 400 Da. Branch erythropoietin daga impurities ne da za'ayi da ion-musayar kuma dangantaka chromatography. Human recombinant EPO yana da wani tsarki na 98%.

Recombinant erythropoietin da analogs daga gare

Don ta da erythropoiesis aiwatar da likitoci amfani da daban-daban kwayoyi:

• "Aranesp".
• "Aeprin".
• "Epobiokrin".
• "Bioein".
• "Vepoks".
• "Binokrit".
• "Epokrin".
• "Gemax".
• "Epogen".
• "Eprex".
• "Epovitan".
• "Epomaks".
• "Giperkrit".
• "Eralfon".
• "Eritrea".
• "Recormon".
• "Epostim".
• "Epozino".
• "Epoetin Beta".

Kafin ka maye gurbin wani recombinant erythropoietin analogues, kamata shawarci likitan.

alamomi

Medical practitioners sau da yawa da za a magance eritropoetindefitsitnymi anemia (Eda). Wannan kungiyar hada da wadannan pathologies:

• anemia of m neoplasms.
• farkon anemia na prematurity toddlers (kafin 34 makonni gestation) tare da jiki nauyi na 750 zuwa 1500 g;
• nephrogenic anemia.
• Anemia a kullum cututtuka (hepatitis C, rheumatoid amosanin gabbai, HIV, alimentary canal cututtuka).

A rarrabe da sama-karanci anemia ne da cewa su ba su bi da baƙin ƙarfe shirye-shirye. Har kwanan nan, kadai tasiri far ne transfussion. Saboda gaskiya cewa irin wannan far yana da wata babbar yawan illa da kuma wani babban hadarin yaduwar jini-haifa pathogens na kawo hadari cututtuka (HIV, hepatitis ƙwayoyin cuta, da dai sauransu), wannan hanya a zamani magani ne kusan ba amfani. Recombinant mutum erythropoietin taimaka wajen inganta ingancin rayuwa ga marasa lafiya da ganewar asali "eritropoetindefitsitnaya anemia." Biosynthesis da gabatarwar recombinant mutum EPO bude wani sabon zamanin da magani daga anemia eritropoetinzavisimyh.

contraindications

Doctors ba da shawarar yin amfani da recombinant erythropoietin (manual yayi kashedin game da shi), a gaban wadannan pathologies:

• thromboembolism.
• hypersensitivity da miyagun ƙwayoyi.
• rashin yiwuwar tasiri anticoagulant far.
• canjawa wuri cerebral bugun jini ko tsokar zuciya infarction.
• m angina.
• uncontrolled hauhawar jini.
• lokaci na haihuwa da kuma shayarwa.
• tsaurin hauhawar jini.

dosing regimen

A sashi da tsawon magani makirci aka kafa a mai tsananin mutum akai, dangane da tsananin daga anemia, da janar yanayin haƙuri da yanayin da Pathology. Da miyagun ƙwayoyi ne aka yi nufi ga parenteral amfani. Harufan allurai na iya bambanta a cikin kewayon daga 50 zuwa 150 Iu / kg. A kashi ya kamata a gyara dangane da haƙuri da shekaru. Recombinant erythropoietin mafi yawa ana gudanar da sau 3 a mako. Idan yawan abin sama da aka kara illa. A sakamakon zama fiye ko žasa da bayyane bayan 2-3 makonni na aikace-aikace.

gefen sakamako

Ka nada recombinant mutum erythropoietin? Umurnai na Amfani bai ware da ci gaban da illa. Su ne:

• myalgia.
• dizziness.
• hyperthermia.
• drowsiness.
• amai.
• ciwon kai.
• zawo.
• arthralgia.
• hauhawar jini.
• ciwon kirji.
• thrombocytosis.
• tachycardia.
• hawan jinni rikicin.
• convulsions.
• steatosis.
• kiwon da matakin na AST, Alt a cikin jini.
• erythrocyte jam cell aplasia.
• eczema.
• angioedema.
• fata rash kuma itching.
• asthenia.
• amya, redness da kuma kona a shafin na gwamnati.
• hyperkalemia.
• akan rage gina jiki taro a jini ferritin.
• hyperphosphatemia.

Erythropoiesis stimulants a wasanni

Kwanan nan, akai-akai amfani da miyagun ƙwayoyi "Epovitan" (recombinant mutum erythropoietin). A yanzu kayan aiki ne sau da yawa amfani a wasanni (guje guje, bodybuilding, iyo, biathlon). Ya ce shirye-shiryen da activates da biosynthesis na ja da maikacin jini, da bi da bi take kaiwa zuwa wani karuwa a ciki da oxygen da naúrar girma na jini, saboda haka, to da karuwa a jini oxygen iya aiki da kuma Yã ₂ bayarwa ga gabobin da kyallen takarda. Wannan inji na mataki qara aerobic jimiri na wani dan wasa. A irin wannan sakamako ne lura lokacin da horar da wani dan wasa a wani Midlands, inda rashin Yã ₂ a cikin iska ya haddasa ci gaban hypoxia, wanda a nuna activates da biosynthesis na na ciki erythropoietin. EPO shirye-shirye da ake amfani da a hade tare da insulin, girma hormone (GH, somatotropin) da kuma stanazolol.

Wuce kima, uncontrolled amfani da "Erythropoietin" miyagun ƙwayoyi a wasanni zai iya sa jini clots cewa yawanci take kaiwa zuwa mutuwa. Gane recombinant EPO ne quite wuya, saboda roba mahadi tsari ne m zuwa ta physiological analog, don haka wannan biosoedinenie har yanzu ba bisa ka'ida ba a yi amfani da high-yi wasanni a matsayin doping.